This advanced manual includes evolving methods for studying dynamic changes in living cells and organisms, presenting techniques as well as background material, and serving also as a text in advanced courses. The first section covers principles and fundamental issues of detection and imaging; the second provides detailed protocols for imaging live systems. Unique to this edition are advances in 3-D microscopy: atomic force microscopy and structured illumination microscopy (using OMX). New analytical options are also described: live high-throughput/content screening and computational analysis of live cell data.

This advanced manual includes evolving methods for studying dynamic changes in living cells and organisms, presenting techniques as well as background material, and serving also as a text in advanced courses. The first section covers principles and fundamental issues of detection and imaging; the second provides detailed protocols for imaging live systems. Unique to this edition are advances in 3-D microscopy: atomic force microscopy and structured illumination microscopy (using OMX). New analytical options are also described: live high-throughput/content screening and computational analysis of live cell data.