This advanced manual includes evolving methods for studying dynamic changes in living cells and organisms, presenting techniques as well as background material, and serving also as a text in advanced courses. The first section covers principles and fundamental issues of detection and imaging; the second provides detailed protocols for imaging live systems. Unique to this edition are advances in 3-D microscopy: atomic force microscopy and structured illumination microscopy (using OMX). New analytical options are also described: live high-throughput/content screening and computational analysis of live cell data....
